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What it is
A women's-transitions formula built around indole-3-carbinol (I3C) and its metabolite DIM (diindolylmethane), the plant compounds formed from cruciferous vegetables like broccoli and kale. These are studied for their role in supporting the liver's normal, healthy metabolism of estrogen through its everyday phase-I and phase-II detoxification pathways. Companion actives such as calcium-D-glucarate and flax lignans may be included; exact per-serving amounts are held as until the current label is verified.
Why the evidence supports it
The mechanism is unusually well mapped for a botanical: human trials of I3C, DIM, and cruciferous-vegetable intake consistently show a shift in estrogen metabolism toward the 2-hydroxyestrone pathway -- the pattern researchers associate with gentler estrogenic activity -- rather than adding or blocking any hormone. The research base is real but still developing, and this formula leans on food-derived compounds rather than a synthetic hormone, which is the honest appeal. That makes it an evidence-based way to support the cell's Adapt function -- the liver's own machinery for processing estrogen -- and it is strictly metabolism support, not a treatment for any hormonal condition; anything diagnosed belongs with your clinician.
What it supports
- Supports the body's own healthy estrogen metabolism
- Supports the liver's normal phase-I and phase-II detoxification pathways
- Provides I3C and DIM, compounds found in cruciferous vegetables
- Complements a diet already rich in cruciferous vegetables
Which function it supports
Ties to the cell's Adapt function -- I3C/DIM support the liver's 2-hydroxylation and methylation-linked detox machinery that processes estrogen, rather than adding or blocking a hormone directly. This is estrogen-metabolism support only, not treatment of any hormonal condition.
Learn the why behind this pillarHow it relates to your cells and mind
Every product here ties back to a specific job your body does. This one touches the functions below -- follow either link to see the mechanism in full, then dig into the studies.
What it is made of -- and the mechanisms behind it
Each ingredient acts on several of the body's functions. Taken together, this product touches these cell and mind functions -- follow any to see the mechanism in full.
The ingredients
Mechanisms drawn from our ingredient knowledge base (6 profiled ingredients). Structure and function only -- not a treatment claim.
The science on the ingredients
These studies are research on the ingredients and mechanisms in this product, not on the finished product itself, and they are shown for education. For this ingredient most of the published work is laboratory or animal research rather than human trials, so read it as biology worth knowing rather than proof of an effect in people. Nothing here is medical advice, and nothing here may be used to diagnose, treat, cure, or prevent any disease. Talk to your own provider about what is right for you.
- Indoles Derived From Glucobrassicin: Cancer Chemoprevention by Indole-3-Carbinol and 3,3'-Diindolylmethane
Current Pharmacology Reports · 2021
This review describes how indole-3-carbinol and its metabolite DIM, both formed from a compound in cruciferous vegetables, activate the aryl hydrocarbon receptor and the Nrf2 antioxidant pathway to help regulate normal cell growth signaling and detoxification enzyme activity, mechanisms distinct from their effects on estrogen metabolism.
- Extraction and Quantification of Sulforaphane and Indole-3-Carbinol from Rapeseed Tissues Using QuEChERS Coupled with UHPLC-MS/MS
Molecules · 2020
A QuEChERS extraction method coupled with UHPLC-MS/MS successfully quantified sulforaphane and indole-3-carbinol in rapeseed tissues at parts-per-billion sensitivity.
- Genotoxicity Studies of Indole-3-carbinol and N-Methoxyindole-3-carbinol -- The Effect of Sulphotransferases
Pharmaceuticals · 2026
Neoglucobrassicin and its breakdown product N-methoxyindole-3-carbinol were highly genotoxic in vitro and formed DNA adducts in mice, strongly enhanced by SULT1A1, while indole-3-carbinol was only weakly mutagenic.
- Indole-3-carbinol ameliorates ER stress-mediated hyperleptinemia in western diet-fed apoE-/- mice.
Food & nutrition research · 2026
Indole-3-carbinol reduced adipose weight, plasma leptin, and ER stress markers while boosting cholesterol efflux and fatty acid oxidation genes, partially easing ER stress in atherosclerotic lesions of western-diet-fed mice.
- Indole-3-carbinol attenuates cisplatin-induced premature ovarian failure by activating Nrf2 through competitive binding of Keap1.
Phytomedicine : international journal of phytotherapy and phytopharmacology · 2026
In mice, indole-3-carbinol activated Nrf2 by competitively binding Keap1, reducing ferroptosis and oxidative damage in ovarian tissue after cisplatin exposure by regulating GPX4, SLC7A11, and NNT redox pathways.
- Indole-3-Carbinol (I3C) suppression of IgG2b-triggered signaling in innate cells involves early suppression of p-Akt.
Toxicology and applied pharmacology · 2026
In an immune signaling study linked to multiple sclerosis research, indole-3-carbinol suppressed IgG2b-triggered Akt phosphorylation in innate immune cells, suggesting a nontoxic anti-inflammatory signaling mechanism worth further study.
Association Between Gut Microbiota and Postoperative Delirium-A Scoping ReviewEmergingExpert review2026
This scoping review of seven studies found that gut microbiota disturbances and related metabolic and immune changes, including altered indole levels, may increase the risk of postoperative delirium in surgical patients.
Izabella Prządo; Patrycja Patronik; Łukasz Karaś; Katarzyna Piekarz; Wioletta Mędrzycka-Dąbrowska; Sabina Krupa-Nurcek
The Aryl Hydrocarbon Receptor and Its Crosstalk: A Chemopreventive Target of Naturally Occurring and Modified PhytochemicalsEmergingExpert review2024
In a review, indole-3-carbinol and resveratrol from plants modulated aryl hydrocarbon receptor signaling linked to cancer-associated cell pathways.
Hanna Szaefer; Barbara Licznerska; Wanda Baer-Dubowska
Genotoxicity Studies of Indole-3-carbinol and N-Methoxyindole-3-carbinol -- The Effect of SulphotransferasesEmerging2026
Neoglucobrassicin and its breakdown product N-methoxyindole-3-carbinol were highly genotoxic in vitro and formed DNA adducts in mice, strongly enhanced by SULT1A1, while indole-3-carbinol was only weakly mutagenic.
Hansruedi Glatt; Fabian Schumacher -- Pharmaceuticals
3,3'-Diindolylmethane attenuates recognition memory impairment induced by binge ethanol exposure in mice.2026
In mice exposed to a binge ethanol protocol, DIM administration reduced ethanol-induced recognition memory deficits, suggesting a neuroprotective effect against alcohol-related cognitive impairment.
Morales-Puerto N; Vidal R; Gil de Biedma-Elduayen L; Giménez-Gómez P; Núñez-de la Calle C; Trueba Y; Pérez-Hernández M; Colado MI; Gutiérrez-López MD; O'Shea E -- Biochemical pharmacology
Photochemical Aging of Indole SOA: Implications for Volatility and Optical PropertiesEmerging2026
Indole secondary organic aerosol aged for 5 days lost nitrogen-containing CHON species and absorbed less light than 1-day aged aerosol, showing significant photobleaching, while heating raised the imaginary refractive index.
Thenoor Chandran Ajith; Diego Calderon-Arrieta; Hongwei Pang; Zheng Fang; JingKai Wang; Jessica Knull; Nyiri Hajian; Kirby Hill; Chunlin Li; Alexander Laskin; Yinon Rudich
I3C protects IPEC-J2 cells by inhibiting ferroptosis through activation of the AhR-Nrf2-SLC7A11-GPX4 pathways.2026
In piglet intestinal epithelial cells, indole-3-carbinol reduced LPS-induced barrier damage by activating the AhR-Nrf2-SLC7A11-GPX4 pathway, which inhibited ferroptosis-related cell death.
Jiang H; Zhang Y; Hu X; Guo C; Shi L; Xun W -- International immunopharmacology
Regulation of allergies across the body by microbial metabolitesEmerging2026
A review describes how microbial short-chain fatty acids, indole metabolites and bile acids act on epithelial cells, dendritic cells, macrophages and lymphocytes to regulate allergy, and strengthen regulatory T cells to promote tolerance.
Chang H Kim; James R Baker
Indole-3-carbinol attenuates cisplatin-induced premature ovarian failure by activating Nrf2 through competitive binding of Keap1.2026
In mice, indole-3-carbinol activated Nrf2 by competitively binding Keap1, reducing ferroptosis and oxidative damage in ovarian tissue after cisplatin exposure by regulating GPX4, SLC7A11, and NNT redox pathways.
Zhu F; Zhang R; He Z; Zhang N; Li F; Li J; Chen H; Liu X; Cheng L; Zhong F -- Phytomedicine : international journal of phytotherapy and phytopharmacology
The Dark Side of Grasslands: Endophyte Toxicosis in Horses-Exposure Risks, Health Consequences, and ManagementEmerging2026
A review reports rising concentrations and co-occurrence of ergot alkaloids and indole diterpenes in fescue and ryegrass pastures during hot arid summers, raising the risk of neurotoxic endophyte toxicosis in horses.
Qendrim Zebeli; Lena Lindner; Barbara U Metzler-Zebeli
Indole-3-carbinol ameliorates ER stress-mediated hyperleptinemia in western diet-fed apoE-/- mice.2026
Indole-3-carbinol reduced adipose weight, plasma leptin, and ER stress markers while boosting cholesterol efflux and fatty acid oxidation genes, partially easing ER stress in atherosclerotic lesions of western-diet-fed mice.
Kim HJ -- Food & nutrition research
Microbiome-Derived Indole-3-Lactic Acid Attenuates Cutibacterium Acnes-Induced Inflammation via the Aryl Hydrocarbon Receptor PathwayEmerging2026
In Cutibacterium acnes-stimulated human keratinocytes and an acne mouse model, indole-3-lactic acid suppressed TNF-alpha, IL-1beta and COX2 and reduced inflammatory infiltration by activating the aryl hydrocarbon receptor.
Lee SG; Chau NH; Ham S; Baek Y; Nguyen NH; Kim SH; Lee YI -- International journal of molecular sciences
3,3'-Diindolylmethane ameliorate obesity-related skeletal muscle atrophy via regulating mitochondrial function.2026
DIM improved obesity-related skeletal muscle atrophy in a high-fat diet model by restoring mitochondrial number and function, likely via the AMPK/SIRT1/PGC-1alpha signaling pathway.
Zhong Y; Chen S; Pan J; Niu R; Du J; Dong Q; Liu Y; Yao Y; Lin Y; Fang H; Su J; Li X; Zhang Y; Yang G; Wu J; Li J; Liu W; Huang B; Tang J; Zhu W -- The Journal of nutritional biochemistry
Endogenous and environmental factors that induce DNA replication defects and genomic instability in ER-negative heterozygous BRCA1 cellsEmerging2026
In heterozygous BRCA1-mutant mammary cells, estrogen metabolites and the herbicide atrazine both stalled replication forks and raised genomic instability, while dietary indole-3-carbinol reduced that replication stress.
Madhura Deshpande; Theodore Paniza; Rebecca Brown; Kate Heslin; Nitya Patel; Advaitha Madireddy; Zev Rosenwaks; Jeannine Gerhardt
The role of indole metabolites derived from gut microbiota in Parkinson's disease: A comprehensive review.2026
Gut dysbiosis toward Enterobacteriaceae redirects tryptophan metabolism away from protective indole compounds like indole-3-carbinol toward toxic intermediates, disrupting barrier function and amplifying neuroinflammation linked to Parkinson's disease.
Meng S -- Brain research bulletin
Gut-microbiota-derived indole sulfate promotes heart failure in chronic kidney diseaseEmergingPrimary research (design unspecified)2025
In a prospective chronic kidney disease cohort, gut-derived indole sulfate promoted heart failure and E. coli abundance showed diagnostic potential for predicting onset within 6 months.
Zhang Y; Han X; Feng T; Li Z; Yu H; Chen Y; Gao Y; Gao Q; Zhang L; Li S; Shi L; Zhang X; Li Z; Li Y; Zhou H -- Cell host & microbe
3,3'-Diindolylmethane ameliorates muscle atrophy by modulating mitochondrial function and calcium homeostasis.2026
In mice and C. elegans, 3,3'-diindolylmethane (DIM) improved grip strength and myofiber quality, reduced mitochondrial dysfunction, and countered dexamethasone-induced muscle atrophy, suggesting a mechanistic approach to addressing sarcopenia.
Gao X; Feng X; Feng G; Zhong J; Liu Y; Pan S; Wang Z; Miao S; Lu R; Sun K; Ye Y -- Phytomedicine : international journal of phytotherapy and phytopharmacology
The impact of 3,3'-diindolylmethane on estradiol and estrogen metabolism in postmenopausal women using a transdermal estradiol patchEmerging2025
Among 1458 postmenopausal women using a transdermal estradiol patch, the 108 also taking a diindolylmethane supplement showed altered urinary estrogen metabolite profiles, suggesting a possible supplement-hormone therapy interaction.
Mark S Newman; Jaclyn Smeaton
Therapeutic Efficacy of Indole-3-Carbinol Against SARS-CoV-2-Induced Acute Respiratory Distress Syndrome: A Dual Antiviral and Anti-Inflammatory Approach in a Golden Syrian Hamster Model.2026
In a hamster model of severe COVID-19, indole-3-carbinol reduced weight loss, lung damage, and pulmonary TNF-alpha levels through combined antiviral and anti-inflammatory action, at a non-toxic dose.
Federica C; Barbara R; Andrea L; Michela B; Marco P; Francesca S; Manuel S; Alessandro M; Palyi B; Deri D; Novak B; Kis Z; Filippone C; Pandolfi PP; Giuseppe N -- Journal of cellular and molecular medicine
Application of Tryptophan and Methionine in Broccoli Seedlings Enhances Formation of Anticancer Compounds Sulforaphane and Indole-3-Carbinol and Promotes GrowthEmergingPrimary research (design unspecified)2024
In broccoli seedlings, combined tryptophan and methionine application increased glucoraphanin 2.37-fold and sulforaphane 1.99-fold with enhanced myrosinase activity.
Rui Li; Zihuan Zhou; Xiaofei Zhao; Jing Li -- Foods
Diindolylmethane confers nootropic and neuroprotection effects against scopolamine-induced brain injury in Wistar rats.2026
In rats given scopolamine to impair memory, diindolylmethane improved cognitive test performance, raised acetylcholine levels, and reduced brain oxidative stress, inflammation, and neuronal apoptosis markers compared to untreated animals.
Anusha VL; Dontiboina HKR; Yadava S; Dumala N; Malothu N; Guntupalli C; Kaurav H; Ramakrishna K -- 3 Biotech
Synthetic Methodologies and Therapeutic Potential of Indole-3-Carbinol (I3C) and Its DerivativesEmerging2023
This review covers synthetic routes to indole-3-carbinol from Brassicaceae vegetables and its reported antitumor and antiviral activity, including inhibition of NEDD4 and WWP1 ubiquitin ligase activity.
Federica Centofanti; Alessandro Buono; Michele Verboni; Carlo Tomino; Simone Lucarini; Andrea Duranti; Pier Paolo Pandolfi; Giuseppe Novelli
Novel 3,3'-diindolylmethane derivatives as multi-pathway modulators: targeting estrogen-dependent and independent breast cancer carcinogenesis.2026
Novel DIM derivatives showed antiestrogenic activity via aromatase and CYP1B1 inhibition and selective cytotoxicity toward estrogen receptor-positive cells, suggesting potential as adjuvant candidates for hormone-dependent breast cancer therapy.
Ince-Erguc E; Entezari B; Sirinzade H; Suzen S; Akdemir A; Erdogan O; Gurer-Orhan H -- Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences
Qualitative Analysis of Cruciferous Vegetable Extracts Useful for Estrogen Metabolism for Diindolylmethane (DIM)EmergingPrimary research (design unspecified)2023
In local market vegetables from Nigeria, HPLC confirmed detectable DIM in broccoli, spinach, and cabbage.
Genotoxicity Studies of Indole-3-carbinol and N-Methoxyindole-3-carbinol-The Effect of Sulphotransferases.2026
Study of indole-3-carbinol and N-methoxyindole-3-carbinol derivatives, some showing anti-carcinogenic activity, examined genotoxicity influenced by sulfotransferase enzymes, noting synthetic N-substituted variants being developed as antineoplastic drugs may vary widely in this safety property.
Glatt H; Schumacher F -- Pharmaceuticals (Basel, Switzerland)
Balanced by design -- we show supporting and contradicting findings. Search all studies
If you have had labs done
These are lab markers where this product's ingredients have measured evidence in our citation library. This is not a claim that the product will change your results -- the research attaches to the ingredient, and what a marker means depends on the whole picture. Each one links to its full entry, including what typically moves it and how to read it.
- Sex Hormone Binding Globulin (SHBG)emerging evidence
via Indole-3-carbinol / DIM · 1 studies, 1 in people
What people often notice when this marker sits outside the usual range
Higher: High SHBG lowers FREE testosterone even when total testosterone reads perfectly normal: low libido, low drive, fatigue, loss of muscle.
Lower: SHBG produces no symptoms of its own -- it changes what the sex hormones do, so the symptoms are theirs.
- Fecal Calprotectinemerging evidence
via Pomegranate extract · 1 studies, 1 in people
A 12-week juice intervention with a modulatory effect on the relevant transcriptional profile.
What people often notice when this marker sits outside the usual range
Higher: Diarrhoea, often with urgency; blood or mucus in stool.
- Estradiol, FSH, LH, Prolactin, DHEA-S & Testosteronemixed evidence
via Black cohosh (Cimicifuga racemosa) · 17 studies, 3 in people
What people often notice when this marker sits outside the usual range
Higher: HIGH FSH with low oestradiol is the menopause transition signal.
Lower: Joint aches that appeared with no injury; skin and hair changing texture.
- Estradiol (E2)mixed evidence
via Black cohosh (Cimicifuga racemosa) · 17 studies, 3 in people
What people often notice when this marker sits outside the usual range
Lower: Hot flushes and night sweats; vaginal dryness and painful sex; poor sleep.
- Estradiol (E2)mixed evidence
via Soy isoflavones (Genistein/Daidzein) · 9 studies, 2 in people
What people often notice when this marker sits outside the usual range
Higher: Breast tenderness, heavy or painful periods, fluid retention, worsening premenstrual mood, headaches around the period.
Lower: Vaginal dryness, painful sex, poor sleep, low mood, hot flushes, brain fog and word-finding difficulty.
Lab results are interpreted by a qualified clinician who knows your history. Nothing here diagnoses anything or replaces that conversation.
No product works in isolation
Every cell function above -- Sense, Exchange, Transform, Build, Maintain, Adapt -- needs the same broad base of optimal micronutrient levels running underneath it, all the time. Estera Phase 2 is not a stand-alone fix; it is one piece that plugs into that larger system. The foundation comes first, and this supports it.
Stacks well with
Pick your goal -- here is what Estera Phase 2 pairs with for each. Nothing works in isolation; these share the same systems.
For Women's Health
For Mood & Hormones
Foundational, or targeted?
Not everything in the shop is meant to be taken forever. Some products here are foundational -- a daily nutrient base built for long-term, ongoing use. Others are targeted -- shorter-term support for a specific season, not a lifelong habit. If you are not sure which one this is, that is a fair question to ask us before you buy.
Learn more, on your terms
Do not take our word for it -- go read. Each topic below opens a pre-loaded search so you can explore the nutrient, the mechanism, or the device for yourself.
Search the full studies libraryHow to use it
Take the labeled daily serving with a meal, following the current bottle directions. This is a structure/function formula, not a hormone treatment, and it is not designed to move a diagnosed hormonal condition in either direction.
Where to get it
Ready when you are. This link takes you to the official, verified source for this product.
Get Estera Phase 2The studies
See the research behind the science we point to.
Explore studiesThe blog
Plain-language reads on how it all fits together.
Read the blogOptimal nutrients are the definition of longevity
Long-term cell function depends on having what it needs, at the level it needs it, for as long as it needs it. That is the whole thesis behind everything we point you to here.
When you buy through our links, we earn a small commission -- and that is what funds all the free education here. It never costs you more. Products are not intended to diagnose, treat, cure, or prevent any disease.
Estera Phase 2 is built around indole-3-carbinol and DIM, the compounds your body forms from cruciferous vegetables like broccoli and kale. It concentrates that cruciferous chemistry into a daily capsule, aimed at women during an active transition.
FAQ
What does Estera Phase 2 support?
It supports the body's normal, healthy metabolism of oestrogen through the same pathways cruciferous vegetables act on, and supports women through the everyday changes of a hormonal transition.
How do I take it?
Take the daily serving with food, per the label. It is the active-transition phase of a two-part family, with Phase III carrying the same support forward afterwards.
What makes it different?
It concentrates the I3C and DIM you would otherwise get from eating a lot of cruciferous vegetables. Worth an honest note: our own evidence review grades cruciferous hormone claims as contested, so the biochemistry is better established than the outcome data.